Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
Identifieur interne : 000424 ( Main/Exploration ); précédent : 000423; suivant : 000425Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
Auteurs : Tatsuya Kato [Japon] ; Yoshihiro Takami [Japon] ; Vipin Kumar Deo [Japon] ; Enoch Y. Park [Japon]Source :
- Journal of Biotechnology [ 0168-1656 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux, Bombyx (génétique), Bombyx (métabolisme), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Dipeptidyl peptidase 4 (métabolisme), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (isolement et purification), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Hémolymphe (métabolisme), Infections à coronavirus (), Infections à coronavirus (diagnostic), Larve (métabolisme), Lignée cellulaire, Matériaux biomimétiques (), Matériaux biomimétiques (métabolisme), Protéines d'insecte (génétique), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (isolement et purification), Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (isolement et purification), Protéines de la matrice virale (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Signaux de triage des protéines (génétique), Tensioactifs (pharmacologie), Vaccins à pseudo-particules virales (), Vaccins à pseudo-particules virales (métabolisme), Vésicules extracellulaires ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Bombyx, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Protéines d'insecte, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines recombinantes, Signaux de triage des protéines.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Glycoprotéine de spicule des coronavirus, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines recombinantes.
- métabolisme : Bombyx, Dipeptidyl peptidase 4, Glycoprotéine de spicule des coronavirus, Hémolymphe, Larve, Matériaux biomimétiques, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines recombinantes, Vaccins à pseudo-particules virales.
- pharmacologie : Tensioactifs.
- Animaux, Humains, Infections à coronavirus, Lignée cellulaire, Matériaux biomimétiques, Vaccins à pseudo-particules virales, Vésicules extracellulaires.
English descriptors
- KwdEn :
- Animals, Biomimetic Materials (chemistry), Biomimetic Materials (metabolism), Bombyx (genetics), Bombyx (metabolism), Cell Line, Coronavirus Infections (diagnosis), Coronavirus Infections (prevention & control), Dipeptidyl Peptidase 4 (metabolism), Extracellular Vesicles (chemistry), Hemolymph (metabolism), Humans, Insect Proteins (genetics), Larva (metabolism), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Protein Sorting Signals (genetics), Recombinant Proteins (genetics), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (isolation & purification), Spike Glycoprotein, Coronavirus (metabolism), Surface-Active Agents (pharmacology), Vaccines, Virus-Like Particle (chemistry), Vaccines, Virus-Like Particle (drug effects), Vaccines, Virus-Like Particle (metabolism), Viral Envelope Proteins (genetics), Viral Envelope Proteins (isolation & purification), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (genetics), Viral Matrix Proteins (isolation & purification), Viral Matrix Proteins (metabolism).
- MESH :
- chemical , chemistry : Vaccines, Virus-Like Particle.
- chemical , drug effects : Vaccines, Virus-Like Particle.
- chemical , genetics : Insect Proteins, Protein Sorting Signals, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , isolation & purification : Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , metabolism : Dipeptidyl Peptidase 4, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Vaccines, Virus-Like Particle, Viral Envelope Proteins, Viral Matrix Proteins.
- chemistry : Biomimetic Materials, Extracellular Vesicles.
- diagnosis : Coronavirus Infections.
- genetics : Bombyx, Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- metabolism : Biomimetic Materials, Bombyx, Hemolymph, Larva.
- chemical , pharmacology : Surface-Active Agents.
- prevention & control : Coronavirus Infections.
- Animals, Cell Line, Humans.
Abstract
Virus-like particle mimetic nanovesicles were prepared using insect cells. Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs. Mechanical extrusion is more effective way to display the S protein on VLPs. S protein on the surface of nanovesicles were confirmed by immuno-TEM. The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.
Url:
DOI: 10.1016/j.jbiotec.2019.10.007
PubMed: 31614169
PubMed Central: 7114102
Affiliations:
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Le document en format XML
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uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, 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(genetics)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (isolation & purification)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Surface-Active Agents (pharmacology)</term><term>Vaccines, Virus-Like Particle (chemistry)</term><term>Vaccines, Virus-Like Particle (drug effects)</term><term>Vaccines, Virus-Like Particle (metabolism)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (isolation & purification)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (isolation & purification)</term><term>Viral Matrix Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bombyx (génétique)</term><term>Bombyx (métabolisme)</term><term>Coronavirus du syndrome 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virale (isolement et purification)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Signaux de triage des protéines (génétique)</term><term>Tensioactifs (pharmacologie)</term><term>Vaccins à pseudo-particules virales ()</term><term>Vaccins à pseudo-particules virales (métabolisme)</term><term>Vésicules extracellulaires ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Vaccines, Virus-Like Particle</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Vaccines, Virus-Like Particle</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Insect Proteins</term><term>Protein Sorting Signals</term><term>Recombinant Proteins</term><term>Spike Glycoprotein, 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coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bombyx</term><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bombyx</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Protéines d'insecte</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Signaux de triage des protéines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Biomimetic Materials</term><term>Bombyx</term><term>Hemolymph</term><term>Larva</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bombyx</term><term>Dipeptidyl peptidase 4</term><term>Glycoprotéine de spicule des coronavirus</term><term>Hémolymphe</term><term>Larve</term><term>Matériaux biomimétiques</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Vaccins à pseudo-particules virales</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Tensioactifs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Infections à coronavirus</term><term>Lignée cellulaire</term><term>Matériaux biomimétiques</term><term>Vaccins à pseudo-particules virales</term><term>Vésicules extracellulaires</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Virus-like particle mimetic nanovesicles were prepared using insect cells.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">Mechanical extrusion is more effective way to display the S protein on VLPs.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">S protein on the surface of nanovesicles were confirmed by immuno-TEM.</p></list-item><list-item id="lsti0025"><label>•</label><p id="par0025">The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, J F" uniqKey="Chan J">J.F. 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Park</name><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y." last="Park">Enoch Y. Park</name><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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